Analysis Metallothionein of Carp fish in The Brantas River, Indonesia

Putri Ayu Ika, Setiyowati and Rofiatun, Solekha (2020) Analysis Metallothionein of Carp fish in The Brantas River, Indonesia. Analysis Metallothionein of Carp fish in The Brantas River, Indonesia, 1 (2). pp. 139-146. ISSN E-ISSN: 2720-9326, P-ISSN: 2716-0459

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Abstraksi

: This study aimed to detection of protein profile, expression of metalothionein (MT) protein, and analyze difference of MT density in liver and gill in one of Carp fish that is Barbonymus balleroides in the upstream and downstream of Brantas river. The method used observasional analytic, Two individual of Barbonymus balleroides samples taken three times (with difference of month) from two station there are upstream and downstream of Brantas river. Analysis protein profile used electrophoresis SDS-PAGE (15%), expression and density of MT used western blot method and imageJ software. Difference between MT density in liver and gill of Barbonymus balleroides in the upstream and downstream of Brantas river analyzed with Two-way ANOVA. The results showed, protein profile in liver and gill Barbonymus balleroides in the upstream and downstream Brantas river have molecular weight about 8-93 kDa, expression of MT showed band of MT with molecular weight 24 kDa, results of MT density in liver and gill on upstream and downstream, continuously 231.29 MT/µm2 and 229.87MT/µm2 , 232.41 MT/µm2 and 231.56 MT/µm2 but there is not significant.

Item Type:	Article
Uncontrolled Keywords:	Brantas river, metallothionein, liver, gills, Carp fish Barbonymus balleroides
Subjects:	Fakultas Sains, Teknologi dan Pendidikan > S1 Biologi
Divisions:	Fakultas Sains, Teknologi dan Pendidikan
Depositing User:	Dosen rofiatun solekha
Date Deposited:	02 Sep 2022 08:58
Last Modified:	02 Sep 2022 08:58
URI:	http://repository.umla.ac.id/id/eprint/2121

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