Antioxidant protective effect of Nelumbo nucifera extract against spermatogenic cells in male mice due to 2-methoxyethanol exposure

Musyarofah, Badriatul (2024) Antioxidant protective effect of Nelumbo nucifera extract against spermatogenic cells in male mice due to 2-methoxyethanol exposure. Jurnal Teknologi Laboratorium. pp. 33-43. ISSN ISSN 2580-0191(Online) / ISSN 2338 – 5634(Print)

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Abstraksi

One of the causes of infertility in men is influenced by the compound 2-Methoxyethanol(2-ME) that can increase Reactive Oxygen Species(ROS) which can distrub spermatogenesis. This study aimed to analyze the effect ofantioxidant ofNelumbonuciferaextract on the histology of mice testicular spermatogenic cells. Male mice are divided into 6 groups. The negative control group (KN) was given distillated for 28 daysad libitum.The positive control group (KP) was injected subcutaneous (s.c) with 2-ME dose 200 mg/kg bw as much as 0.05 ml/ day for 7 days, the drug group (KO) was injected s.c with 2-ME (200 mg/kg bw) as much as 0.05 ml/ day for 7 days and then injecteds.cwith Clomiphene citratewith a dose 50 mg/kg bwas much as 0.2 ml/ day for 21 days. The treatment group (P1, P2, P3) was injecteds.cwith 2-ME (200 mg/kg bw) as much as 0.05 ml/ day for 7 days and injected s.c with N. nucifera extract at low dose 50 mg/kg bw (P1), mediumdose 150 mg/kg bw (P2), and high dose 450 mg/kg bw (P3) as much as 0.2 ml/ day for 21 days. At the end of the study all mice were sacrificed and testicular collection was carried out. Testicular tissue was processed using Hematoxylin-Eosin staining and observed spermatogenic cells (spermatogonia, spermatocytes and spermatids). Data analysis using ANOVA test and advanced test Post hoct test.The results showed that there was a significant difference (p < 0.05) in number of spermatogenic cells of mice testicles between the negative group (KN), positive group (KP) and treatment group (P1, P2, P3). The optimal dose of N. nuciferaextract that is most able to repair testicular tissue damage is a high dose

Item Type:	Article
Uncontrolled Keywords:	Antioxidant; Histopatology; Nelumbo nucifera; Spermatogenic cells; 2- Methoxyethanol
Subjects:	Fakultas Sains, Teknologi dan Pendidikan > S1 Biologi
Divisions:	Fakultas Sains, Teknologi dan Pendidikan
Depositing User:	Badriatul Musyarofah
Date Deposited:	20 Aug 2024 02:47
Last Modified:	20 Sep 2024 07:43
URI:	http://repository.umla.ac.id/id/eprint/4551

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