PREPARATION AND ANTIFUNGAL ACTIVITIES OF CHITOSAN FROM APPLE-SNAIL (Pila ampullacea) SHELLS AS PRESERVING AGENT FOR POSTHARVEST PODANG MANGO (Mangifera indica L.) cv Podang

Aisyah, Ramadani (2022) PREPARATION AND ANTIFUNGAL ACTIVITIES OF CHITOSAN FROM APPLE-SNAIL (Pila ampullacea) SHELLS AS PRESERVING AGENT FOR POSTHARVEST PODANG MANGO (Mangifera indica L.) cv Podang. fakultas Sains, Teknologi dan Pendidikan Universitas Muhammadiyah Lamongan, Universitas Muhammadiyah Lamongan.

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Abstraksi

Abstract: Podang mango, an endemic mango from Kediri, has a short postharvest shelf life that is accelerated by physiological factors and pathogenic fungal contamination. One of the commonly used strategies to preserve the shelf life of the fruit is by using the coating technique. Chitosan has been reported as an active compound for coating and it can be obtained from the apple- snalshel The purpose of this study was to prepare the chitosan from the shells of Pila ampullacea then examined antifungal activities against Fusarium oxysporum and Aspergillus ochraceus, and verified the effect of chitosan coating on shelf life and quality of Podang mango. The chitosan was prepared by transforming chitin in the snail shells to chitosan using demineralization, deproteinization, and deacetylation method. The effects of chitosan coating on fruit quality were analyzed by measuring the physical and chemical characteristics of fruit after coated with chitosan compare to the uncoated. The addition of gelatin was also tested to improve the effect of chitosan. Results showed that chitosan prepared from P. ampullacea had 53.56 degrees of deacetylation. Antifungal activity test of 2% chitosan had the highest inhibitory activity against F.oxysporum and A.ochraceus with 14.46 mm and 10.33 mm inhibition zone. Coating with chitosan statistically affected fruit quality for 14 days of storage (sig <0.05). Chitosan-gelatin coating is the best treatment in reducing weight loss (8.97%), maintain aroma (sweet fragrance), texture (soft), water content (84%), and vitamin C (0.182%), while the color (reddish yellow) was more influenced by chitosan coating. It can be concluded that chitosan is successfully synthesized from apple-snail shells and shows potential antifungal activities, hence, is recommended for application on Podang mangoes. Keywords: chitosan; antifungal; podang mango; snail Indonesian Journal of Applied

Item Type:	Other
Subjects:	Fakultas Sains, Teknologi dan Pendidikan > S1 Biologi
Divisions:	Fakultas Sains, Teknologi dan Pendidikan
Depositing User:	Admin
Date Deposited:	25 Mar 2022 08:53
Last Modified:	25 Mar 2022 08:53
URI:	http://repository.umla.ac.id/id/eprint/1817

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